کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131242 | 1490883 | 2017 | 9 صفحه PDF | دانلود رایگان |
- We provide a facile one step synthesis of hydrophilic PAA-Ti/TiO2 nanocomposite.
- This novel nanocomposite possesses remarkable selectivity for phosphopeptides.
- Abundant titanium is available for selective enrichment of phosphopeptides.
- Plenty of carboxylate groups are present to inhibit nonspecific absorption.
Highly selective enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel affinity material has been synthesized to improve the enrichment specificity for phosphopeptides by using PAAS as coupling molecule. In the resulting materials, highly abundant titanium is available for selective enrichment of phosphopeptides, with plenty of carboxylate groups that can inhibit nonspecific adsorption. The enrichment results demonstrated that the hydrophilic PAA-Ti/TiO2 composite possesses excellent selectivity for phosphopeptides even at a very low molar ratio of phosphopeptides/non-phosphopeptides (1:1000), extreme sensitivity (the detection limit was at the fmol level), and high recovery of phosphopeptides (as high as 78%). Moreover, the as-prepared nanocomposite provides effective enrichment of phosphopeptides from real samples (mouse liver), showing great potential in the detection of low-abundance phosphopeptides in biological samples.
Journal: Analytica Chimica Acta - Volume 949, 1 January 2017, Pages 67-75