کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132075 | 1491477 | 2017 | 12 صفحه PDF | دانلود رایگان |
A biocatalatic pathway for the determination of peroxidase using N, Nâdiethylâp-phylenediamine sulphate (DPD) and 3-amino phenol (3-AP) is presented. The assay is based on the enzymatic consumption of hydrogen peroxide using DPD- 3AP system to give an intense blue colored compound with absorbance maxima at 660â¯nm. The increase in absorbance is proportional to the concentration of peroxidase in the range from 1.5-15.15â¯nM and 0.47 and 15.15â¯nM from rate and fixed time method respectively. The assay was adapted for the measurement of H2O2 at concentrations of 3.5-120 µM. The kinetic parameters like catalytic power, catalytic efficiency, catalytic constant (kcat) and specificity constant (kcat/Km) was found to be 9.78â¯Ãâ¯10â5 µMâ1 minâ1, 1.483â¯Ãâ¯10â3 minâ1, 0.0245â¯Ãâ¯103 minâ1 and 0.0445 µMâ1  minâ1 respectively. The applicability and thermal properties of the method has been tested in different seeds of fruits extracts that showed peroxidase activity.
106
Journal: Chemical Data Collections - Volumes 11â12, December 2017, Pages 84-95