کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135181 1493427 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polymer-modified fibrous mesoporous silica nanoparticles as coating material for open-tubular capillary electrochromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Polymer-modified fibrous mesoporous silica nanoparticles as coating material for open-tubular capillary electrochromatography
چکیده انگلیسی


- A new stationary phase of P-fSiO2 was immobilized to fabricated open tubular column.
- The process of column preparation was convenient and easy-to- handle.
- Both electrophoretic and chromatographic mechanisms were demonstrated in the OT-CEC separation of proteins.

A novel fibrous mesoporous silica nanoparticles (fSiO2) stationary phase grafted with polymer (Poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) was developed for open tubular capillary electrochromatography (OT-CEC). The preparation procedure included synthesizing fSiO2 through biphase stratification approach, removing the surfactants, silanization and in situ graft polymerization with monomers via atom transfer radical polymerization (ATRP). Subsequently, PDMAEMA-modified mesoporous silica nanoparticles (P-fSiO2)/ethanol solution was immobilized onto the inner surface of the pretreated capillary and functionalized with octadecylsilane to fabricate the open-tubular column. Separation of polycyclic aromatic hydrocarbons (PAHs) and proteins were carried out to evaluate the performance of the column in CEC. The run-to-run, day-to-day and column-to-column reproducibility in terms retention time of naphthalene was 1.9%, 2.2%, and 3.7%, respectively. The effects of solvent concentration and pH on the separation were evaluated. The method was also used for the separation of real bio-sample, egg white proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1499, 26 May 2017, Pages 196-202
نویسندگان
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