Suppressive effect of triterpenoids and a flavonol glycoside in seaberry extract on carbacol-induced contraction of bladder smooth muscle and TGF-β-induced contraction of collagen gel containing bladder smooth muscle cells

Moderate urinary dysfunctions including frequent urination and over reactive bladder are age-related deteriorating urinary condition and saw palmetto extract and pumpkin seed oil have been used for the prevention. In the course of evaluation of activities against urinary dysfunctions in edible fruits, we found that extract of seaberry (SBE) suppressed bladder constriction. In carbacol-induced constriction in rat bladder specimen, SBE (10 μg/mL) significantly suppressed the constriction. SBE also suppressed TGF-β-induced constriction of collagen gel containing bladder muscle cells. Among the constituents in SBE, ursolic acid (1-100 μM) and isorhamnetin 7-O-rhamunoside (10 μM) significantly suppressed carbacol-induced bladder constriction. On the other hand, 3-O-coumaroyl 2,23-dihydroxy oleanolic acid, ursolic acid, uvaol, pomolic acid, oleanolic aldehyde and isorhamnetin 7-O-rhamnoside significantly suppressed TGF-β-induced collagen gel constriction containing bladder muscle cells at 1 or 10 μM. These results suggest that several triterpenoids and a flavonoid glycoside suppressed bladder constriction and SBE may contribute to suppression of urinary dysfunctions caused by overactive bladder.