کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5139572 1495182 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy
چکیده انگلیسی


- Conformational disorder has been imaged for the first time in a multilamellar vesicle by Raman microspectroscopy.
- Structural variations, the intra- and inter chain interactions, helped to identify phase and packing efficiencies.
- Non-uniform molecular interactions observed in multilamellar vesicles.
- Gel phase rich domains and heterogeneous lateral packing within a giant liposome has been imaged.
- Our method can help to understand structural deformations induced by external factors such as pH, temperature etc.

Liposomes are closed phospholipid bilayer systems that have profound applications in fundamental cell biology, pharmaceutics and medicine. Depending on the composition (pure or mixture of phospholipids, presence of cholesterol) and preparation protocol, intra- and inter-chain molecular interactions vary leading to changes in the quality (order and packing) of liposomes. So far it is not possible to image conformational disorders and packing densities within a liposome in a straightforward manner. In this study, we utilized confocal Raman microspectroscopy to visualize structural disorders and packing efficiency within a giant multilamellar liposome model by focusing mainly on three regions in the vibrational spectrum (CC stretching, CH deformation and CH stretching). We estimated properties such as trans/gauche isomers and lateral packing probability. Interestingly, our Raman imaging studies revealed gel phase rich domains and heterogeneous lateral packing within the giant multilamellar liposome.

Graphical Abstract397

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 187, 5 December 2017, Pages 186-190
نویسندگان
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