کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5145885 | 1497343 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for hydrogenase-4 catalyzed biohydrogen production in Escherichia coli
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Biohydrogen production by Escherichia coli during fermentation of the mixture of glycerol, glucose and formate at different pH values was studied. Employing mutants lacking large subunits of different hydrogenases (Hyd), it was reported that, at pH 7.5, H2 production was produced except in a hyaB hybC hycE triple mutant, thus suggesting compensatory H2-producing functions of the Hyd enzymes. Activity of Hyd-4 was revealed in glucose assays at pH 7.5 in the triple mutant whereby 62% of the wild type level of H2 production was derived from Hyd-4. In formate assays, it was shown, that, first, the hyaB hybC double mutant had a H2 production â¼3 fold higher than wild type, indicating that Hyd-1 and Hyd-2 oxidize H2, and second, that at pH 5.5, Hyd-4 and Hyd-3 were responsible for H2 production. These findings are significant when applying various carbon sources such as sugars, alcohol and organic acids for biohydrogen production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 34, 24 August 2017, Pages 21697-21703
Journal: International Journal of Hydrogen Energy - Volume 42, Issue 34, 24 August 2017, Pages 21697-21703
نویسندگان
Satenik Mirzoyan, Pablo Maria Romero-Pareja, Maria Dolores Coello, Armen Trchounian, Karen Trchounian,