Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase - Homology model, reactivity and isoenzymatic diversity

Catalytic subunit of steroid C25 dehydrogenase catalyzing regioselective hydroxylation of ketosteroid substrate to 25-hydroxy alcohol using water molecule as an oxygen source. The rate limiting step is the homolytic CH bond cleavage by the oxo ligand (OX) of the molybdenum cofactor (Moco).150