کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5157799 1500601 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biological and structural analyses of bovine heparin fractions of intermediate and high molecular weight
ترجمه فارسی عنوان
تجزیه و تحلیل بیولوژیکی و ساختاری فراکسیون های هپارین گاو وزن مولکولی متوسط ​​و بالا
کلمات کلیدی
هپارین گاو، فوق العاده تصفیه فعالیت ضد انعقاد ترومبوز وریدی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی
Low molecular weight heparin, which is generally obtained by chemical and enzymatic depolymerization of unfractionated heparin, has high bioavailability and can be subcutaneously injected. The aim of the present investigation was to fractionate bovine heparin using a physical method (ultrafiltration through a 10 kDa cut-off membrane), avoiding structural modifications that can be caused by chemical or enzymatic treatments. Two fractions with different molecular weights were obtained: the first had an intermediate molecular weight (B-IMWH; Mn = 9587 Da) and the other had a high molecular weight (B-HMWH; 22,396 Da). B-IMWH and B-HMWH have anticoagulant activity of 103 and 154 IU/mg respectively, which could be inhibited by protamine. Both fractions inhibited α-thrombin and factor Xa in vitro and showed antithrombotic effect in vivo. Moreover, ex vivo aPTT assay demonstrated that B-IMWH is absorbed by subcutaneous route. The results showed that ultrafiltration can be used to obtain two bovine heparin fractions, which differ on their molecular weights, structural components, anticoagulant potency, and administration routes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 157, 10 February 2017, Pages 72-78
نویسندگان
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