New bioactive natural products from Launaea nudicaulis

► We isolated four new compounds from extract of Launaea nudicaulis. ► Structures were established based on 1D, 2D NMR techniques and high-resolution mass spectrometry. ► The structure of compound 4 has also been confirmed through single X-ray crystallography. ► The new compounds have been evaluated for DPPH free radical scavenging and enzyme inhibitory activities.