Possible target-related proteins of salvianolic acids in rat platelets

Salvianolic acids (SAs) extracted from Salvia miltiorrhiza could inhibit ADP-induced aggregation of rat washed platelets. To elucidate the cellular mechanism of the inhibitive effect on platelet aggregation, a proteomic method was used to find the possible target-related proteins of SA in platelets. Treatment of 150 μg/ml SA caused the regulation of platelet levels of 12 proteins, which play important roles in calcium homeostasis, antioxidant system and cytoskeleton structure. The proteomic result was also confirmed by Western blotting.

We report the result of identifying the possible target-related proteins of salvianolic acids (SAs) in rat platelets. Twelve proteins that might be related to the inhibitive effect of salvianolic acids on platelet aggregation were identified.