کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5208153 | 1382552 | 2014 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing
ترجمه فارسی عنوان
طراحی مواد پلیمری برای کشت سلول های بنیادی پلوروپتوف انسانی: پیشرفت در زمینه تولید فیدر و بدون چربی
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کلمات کلیدی
بیومتریک، سلول های بنیادی جنینی، ماتریکس غیر سلولی، فرهنگ سلولی، هیدروژل، میکرو حامل، میکرو کپسول،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
This review describes recent developments regarding the use of natural and synthetic polymers to support the propagation of human pluripotent stem cells (hPSCs), human embryonic stem cells (hESCs), and induced pluripotent stem cells (hiPSCs) while maintaining pluripotency in feeder-free and xeno-free cultures. The development of methods for culturing these cells without using mouse embryonic fibroblasts (MEFs) as a feeder layer will enable more reproducible culture conditions and reduce the risk of xenogenic contaminants, thus increasing the potential clinical applications of differentiated hPSCs. Human or recombinant fibronectin, laminin-511, and vitronectin, which are components of the extracellular matrix (ECM), have been used instead of Matrigel for the feeder-free growth of undifferentiated hPSCs. Successful hPSC cultures have been described for the following conditions: on oligopeptide-immobilized surfaces derived from vitronectin, on microcarriers prepared from synthetic polymers, and encapsulated within three-dimensional (3D) hydrogels composed of alginate and other hydrophilic natural polymers. Recently, synthetic biomaterials that allow hPSCs to maintain pluripotency by secreting endogenous ECM components have been designed. The combination of human ECM proteins or cell adhesion molecules (e.g., oligopeptides and poly-d-lysine) and synthetic biomaterials with well-designed surfaces and/or structures (e.g., scaffolds, hydrogels, microcarriers, microcapsules, or microfibers) in the presence of a chemically defined medium containing recombinant growth factors would offer a xeno-free alternative to feeder cells for culturing hPSCs and maintaining their pluripotency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Polymer Science - Volume 39, Issue 7, July 2014, Pages 1348-1374
Journal: Progress in Polymer Science - Volume 39, Issue 7, July 2014, Pages 1348-1374
نویسندگان
Akon Higuchi, Qing-Dong Ling, S. Suresh Kumar, Murugan Munusamy, Abdullah A. Alarfajj, Akihiro Umezawa, Gwo-Jang Wu,