کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5372305 | 1503983 | 2007 | 11 صفحه PDF | دانلود رایگان |
In this article we use the published heat capacity data of Dragan et al. [A.I. Dragan, et al., The energetics of specific binding of AT-hooks from HMGA1 to target DNA, J. Mol. Biol. 327 (2003) 393-411] on the association of proteins with DNA duplexes to construct enthalpy probability distributions for the protein/DNA complexes formed in these systems. We first analyze the multistep equilibrium that determines the species concentrations in this system to determine whether or not the DNA-peptide complex goes cleanly to DNA single-strands and peptide. Using the heat capacity data for this case we employ the maximum-entropy method to construct enthalpy probability distribution functions for the species involved in this equilibrium. We find that the distribution functions for this system clearly show bimodal behavior indicating a two-state transition from complex to non-complex form.
Journal: Biophysical Chemistry - Volume 125, Issues 2â3, February 2007, Pages 497-507