کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434254 1509140 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrospun vein grafts with high cell infiltration for vascular tissue engineering
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Electrospun vein grafts with high cell infiltration for vascular tissue engineering
چکیده انگلیسی


- This study indicates an effective method for the fabrication of vascular grafts for vessel transplantation.
- The vascular grafts consisting of a densely packed aligned internal layer and a highly porous external layer.
- The bilayer scaffolds were evaluated by in vivo experiments, molecular biology, and histology studies.
- The grafts can enhance the growth of endothelial cells and promoting cell infiltration and tissue growth.

Demand is increasing for functional small-diameter vascular grafts (diameter < 6 mm) for clinical arterial replacement. In the present study, we develop a bilayer poly(ε-caprolactone, PCL) fibrous vascular graft consisting of a thin internal layer made of longitudinally aligned fibers and a relatively thick highly porous external layer. The internal layer provides a scaffold with the necessary mechanical strength and enhances the growth of endothelial cells, whereas the external layer enhances cell motility through the scaffold bulk. The biocompatibility and biological performance of bilayer fibrous scaffolds are evaluated by in vivo experiments, molecular biology, and histology studies. Our bilayer scaffolds demonstrate much better fiber alignment and higher porosity than do normal electrospun vascular grafts with randomly distributed fibers. The results suggest that the proposed grafts can overcome limitations owing to the inadequate porosity, small pores, and poor cell infiltration of scaffolds fabricated by conventional electrospinning. The unique structure of bilayer scaffolds is satisfactory and promotes cell proliferation, collagen-fiber deposition, and ingrowth of smooth muscle cells and endothelial cells in vivo. The results of this study illustrate the strong potential of such bilayer fibrous scaffolds for vascular tissue engineering and regeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 81, 1 December 2017, Pages 407-415
نویسندگان
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