Detection of alprazolam with a lab on paper economical device integrated with urchin like Ag@ Pd shell nano-hybrids

- EμPAD offers many advantageous features such as facile approach, economical and have potential for commercialization.
- Extensive development can be made for industrial translation of this fabricated device.
- Ag@ Pd shell nanohybrids modified EμPAD showed wide linear range 1 - 300 ng/ml and low detection limit of 0.025 ng/l for alprazolam detection.
- The developed sensor was tested in real time samples like urine and serum and found good correlation (99%).

We present results of the studies relating to fabrication of a microfluidic biosensor chip based on urchin like Ag@ Pd shell nano-hybrids that is capable of sensing alprazolam through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of alprazolam present in buffer solutions at clinically relevant concentrations. Methylene blue (MB) was also doped as redox transition substance for sensing alprazolam. Nano-hybrids modified EμPAD showed wide linear range 1-300 ng/ml and low detection limit of 0.025 ng/l. Low detection limit can further enhance its suitability for forensic application. Nano-hybrids modified EμPAD was also employed for determination of drug in real samples such as human urine. Reported facile lab paper approach integrated with urchin like Ag@ Pd shell nano-hybrids could be well applied for the determination of serum metabolites.

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