کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434410 1509143 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Live-cell biosensor for assessment of adhesion qualities of biomaterials
ترجمه فارسی عنوان
بیوسنسور سلول زنده برای ارزیابی کیفیت چسبندگی مواد زیستی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- A live-cell fluorescent biosensor for general assessment of surface biocompatibility
- A standardizing “adhesion quality map”, describing biosensor behavior on natural substrates
- Use of biosensor to demonstrate superior biocompatibility of PDMS-PAA over PDMS-PVP
- Use of biosensor to identify adhesive parameters of PDMS-PAA for further improvement

The present study describes a live-cell biosensor, suitable for general evaluation of adhesion qualities of different substrates. It is based on NIH/3T3 fibroblast cell line stably expressing fusion fluorescently tagged proteins mCherry-vinculin and GFP-tensin as quantifiable markers for assessment not only of focal but also of fibrillar contacts. Four measurable parameters - spreading, polarization and development of focal and fibrillar adhesions were used to standardize the adhesion of biosensor cells after plating on five substrates of natural origin - fibronectin, vitronectin, laminin-111, laminin-521 and collagen type I. The obtained set of values (adhesion quality map) were utilized to describe the default biosensor behavior and as a standard for evaluation of surface biocompatibility of materials with unknown adhesive properties. To demonstrate the applicability of the biosensor we studied two PDMS-based artificial materials. The results demonstrated the superior adhesive properties of the poly(acrylic acid)-containing polymer (PDMS-PAA) over that of poly(vinyl pyrrolidone) copolymer (PDMS-PVP), and pointed out the formation of focal adhesions as a parameter for possible further improvements.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 78, 1 September 2017, Pages 230-238
نویسندگان
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