کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5434512 | 1509144 | 2017 | 14 صفحه PDF | دانلود رایگان |
- Oriented microstructure produced by freeze casing technique.
- Unidirectional microstructure of freeze-cast scaffolds improved physicochemical and mechanical properties.
- Addition of gelatin to PLGA scaffolds increased diameter of pores and cellular spreading.
- Neural differentiation and gen expression of P19 cells observed in freeze-cast retinoic acid-loaded scaffolds.
Different manufacturing processes of scaffolds affect their main properties by altering the microstructure of pores. In this study, poly (lactic-co-glycolic acid) (PLGA) scaffolds were synthesized by both freeze casting and freeze drying methods. Scanning electron microscopy (SEM) micrographs demonstrated a unidirectional microstructure in the freeze-cast and a number of random pores in the freeze-dried scaffolds. According to the results, the formation of a lamellar microstructure increased the mechanical strength, swelling ratio, biodegradation behavior and drug release level. Addition of gelatin to the PLGA freeze-cast constructs led to an increase in the average diameter of pores, hydrophilicity and cellular spreading. The gelatin-containing scaffolds showed a decreased mechanical strength, but at the same time, an enhanced swelling ratio, biodegradation rate and drug release level. Differentiation of P19 cells and expression of β-tubulin III, Pax-6 and Nestin were improved by incorporating retinoic acid in PLGA-Gelatin freeze-cast scaffolds. This is a good option with initial necessary features for regenerating peripheral nervous system (PNS).
198
Journal: Materials Science and Engineering: C - Volume 77, 1 August 2017, Pages 159-172