کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434581 1509144 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical sensing of doxorubicin in unprocessed whole blood, cell lysate, and human plasma samples using thin film of poly-arginine modified glassy carbon electrode
ترجمه فارسی عنوان
سنجش الکتروشیمیایی دکسوروبیسین در خون کلی خون، لسیت سلولی و نمونه های انسانی با استفاده از فیلم نازک الکترود کربن شیشه ای اصلاح شده پلی-آرژینین
کلمات کلیدی
دوکسوروبیسین، الکتروشیمی، مایعات بیولوژیک، پلی آمینو اسید، خون، سلول لیزاتی،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- Thin film of PARG was prepared using electropolymerization methods.
- The performance of PARG toward detection of DOX was investigated.
- DOX was detected in low potential with excellent sensitivity and selectivity.

A thin film of poly-arginine fabricated on glassy carbon electrode by one step electrodeposition method is applied for detection of doxorubicin hydrochloride in whole blood, cell lysate, and untreated-plasma samples. Cyclic voltammetry results indicated that the doxorubicin is oxidized via two electrons and two protons at physiological pH (pH = 7.4) using poly-arginine thin film modified glassy carbon. More importantly, electrostatic repulsion takes place between the prepared polymer film-modified electrode and selected drug resulting in the signal amplification on oxidation of doxorubicin and lowering its over potential and thereby selective detection of doxorubicin in real samples. The apparent electron transfer rate constant and transfer coefficient were determined by cyclic voltammetry and were approximately 10.1 s− 1 and 0.82, respectively. Also, using differential-pulse voltammetric technique for sensitive detection of doxorubicin in whole blood and plasma samples, the lower limit of quantification was 69 nM and 103 nM, respectively. Also, application of this amino acid based biocompatible polymeric electrode was tested to the determination of doxorubicin in unprocessed whole blood and the results show that this sensor could be applied in online and real time monitoring of this anti-cancer drug in real samples which is important for clinical research.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 77, 1 August 2017, Pages 790-802
نویسندگان
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