Review ArticleAssay of superoxide dismutase activity in a plate assay using WST-1

- A robust assay for SOD activity is presented.
- The assay is suitable for SOD measurements on a large number of samples.
- Caveats are described to avoid pitfalls.

One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.

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