کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505449 | 1400269 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for transforming growth factor β1-enhanced invasion of Panc1 cells in vitro
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کلمات کلیدی
TGFβPFKFBPFKFB3F2,6BP6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatasetransforming growth factor-β - تبدیل فاکتور رشد βTransforming growth factor β1 - تبدیل فاکتور رشد β1EMT - تکنسین فوریتهای پزشکیSnail - حلزونFructose 2,6-bisphosphate - فروکتوز 2،6-بیسفسفاتEpithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمیGlycolysis - گلیکولیز یا قندکافت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transforming growth factor β1 (TGFβ1) is a well-established inducer of the epithelial-mesenchymal transition (EMT) that is essential for the acquisition of malignant properties, such as invasion, in tumor cells. Although recent studies suggest that the EMT in tumor cells is associated with reprogramming of energy metabolism and TGFβ1 has been shown to stimulate glycolysis in multiple primary cell lines, little is known about TGFβ1's effect on glycolysis and glycolytic regulators in transformed cells. Given the known regulatory role of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 (PFKFB3) in glycolysis and association of glycolytic activity with malignant features such as invasion, we sought to investigate whether TGFβ1 regulates PFKFB3 expression and if PFKFB3 is involved in the TGFβ1-mediated increase in the invasive ability of the Panc1 cell cline-a well-established model of TGFβ1-initiated EMT. Herein we demonstrate that TGFβ1 induces PFKFB3 expression and stimulates glycolysis in Panc1 cells. We also show that siRNA silencing of PFKFB3 prevents the stimulation of glycolysis and in vitro invasive ability of Panc1 cells by TGFβ1. Furthermore, PFKFB3 silencing suppresses the TGFβ1-mediated induction of the Snail protein, suggesting that PFKFB3 is required for the regulation of Snail expression by TGFβ1. Taken together, our study identifies PFKFB3 as a key TGFβ1 effector protein that mediates TGFβ1's effect on Snail expression, invasion, and glycolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 3, 11 March 2017, Pages 687-693
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 3, 11 March 2017, Pages 687-693
نویسندگان
Abdullah Yalcin, Tugba H. Solakoglu, Selahattin C. Ozcan, Saime Guzel, Sabire Peker, Serap Celikler, Basak D. Balaban, Elif Sevinc, Yunus Gurpinar, Jason A. Chesney,