کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5505554 1400273 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of Deep Vent DNA polymerase
ترجمه فارسی عنوان
ساختار بلوری دی ان ای پلیمراز عمیق
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- The crystal structure of Deep Vent DNA polymerase was determined.
- The structure explains the specificities of DNA polymerases for unnatural base pairs.
- The structure will contribute to the development of mutants for more unnatural bases.

DNA polymerases are useful tools in various biochemical experiments. We have focused on the DNA polymerases involved in DNA replication including the unnatural base pair between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px). Many reports have described the different combinations between unnatural base pairs and DNA polymerases. As an example, for the replication of the Ds-Px pair, Deep Vent DNA polymerase exhibits high efficiency and fidelity, but Taq DNA polymerase shows much lower efficiency and fidelity. In the present study, we determined the crystal structure of Deep Vent DNA polymerase in the apo form at 2.5 Å resolution. Using this structure, we constructed structural models of Deep Vent DNA polymerase complexes with DNA containing an unnatural or natural base in the replication position. The models revealed that the unnatural Ds base in the template-strand DNA clashes with the side-chain oxygen of Thr664 in Taq DNA polymerase, but not in Deep Vent DNA polymerase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 1, 29 January 2017, Pages 52-57
نویسندگان
, , , ,