کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5507039 | 1536897 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world
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کلمات کلیدی
2D-PAGEFAKDNA methyltransferase 1IEFKinomicsCaMKDclkMecp2CDKL5CNBrDnmt1cyclin-dependent kinase-like 5 - 5-کیناز وابسته به سیکلینIPG - GPAimmobilized pH gradient - gradient pH immobilizedMAPK - MAPKMonoclonal antibody - آنتی بادی مونوکلونالtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیcyanogen bromide - برومید سایانوژنisoelectric focusing - تمرکز ذره ای الکتریکیProtein phosphorylation - فسفوریلاسیون پروتئینProteomics - پروتئومیکسProtein kinase - پروتئین کینازmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenCa2+/calmodulin-dependent protein kinase - پروتئین کیناز وابسته به کلسیم و پتاسیم Ca2 + /focal adhesion kinase - کیناز چسبندگی کانونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diverse biological events are regulated through protein phosphorylation mediated by protein kinases. Some of these protein kinases are known to be involved in the pathogenesis of various diseases. Although 518 protein kinase genes were identified in the human genome, it remains unclear how many and what kind of protein kinases are expressed and activated in cells and tissues under varying situations. To investigate cellular signaling by protein kinases, we developed monoclonal antibodies, designated as Multi-PK antibodies, that can recognize multiple protein kinases in various biological species. These Multi-PK antibodies can be used to profile the kinases expressed in cells and tissues, identify the kinases of special interest, and analyze protein kinase expression and phosphorylation state. Here we introduce some applications of Multi-PK antibodies to identify and characterize the protein kinases involved in epigenetics, glucotoxicity in type 2 diabetes, and pathogenesis of ulcerative colitis. In this review, we focus on the recently developed technologies for kinomics studies using the powerful analytical tools of Multi-PK antibodies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 11, September 2017, Pages 40-45
Journal: Biochemistry and Biophysics Reports - Volume 11, September 2017, Pages 40-45
نویسندگان
Yasunori Sugiyama, Isamu Kameshita,