کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5510690 1539311 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Global gene expression analysis of the muscle tissues of medaka acclimated to low and high environmental temperatures
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Global gene expression analysis of the muscle tissues of medaka acclimated to low and high environmental temperatures
چکیده انگلیسی
Medaka (Oryzias latipes) is a temperate eurythermal fish that is able to survive over a wide range of water temperatures ranging from near zero to over 30 °C throughout the year; it maintains its normal physiological and biochemical processes through temperature acclimation. To determine the mechanisms involved in temperature acclimation of fish, the fast skeletal muscle tissues of medaka underwent global gene expression analysis using next-generation sequencing. Ten individuals were placed into two aquariums at 24 °C. While the water temperature of one aquarium was decreased to 10 °C, that of the other aquarium was increased to 30 °C; these temperatures were subsequently maintained for 5 weeks. RNA sequencing (RNA-Seq) analyses revealed that 11 genes involved in energy metabolism and muscle atrophy were significantly highly expressed in the 10 °C-acclimated fish. Meanwhile, significantly higher expression levels were observed for 20 genes encoding myofibrillar proteins and heat shock proteins in the 30 °C-acclimated fish. Moreover, 1103 genes had at least fourfold differential expression between the acclimation groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses provided important information: although the expression of genes related to metabolic processes were activated, muscle atrophy occurred in the 10 °C-acclimated fish, and muscle cells divided actively in the 30 °C-acclimated fish and avoided thermal stress by expressing heat shock proteins. Therefore, RNA-Seq analyses with the available genome database will be useful for better understanding the molecular mechanisms involved in the temperature acclimation of fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 24, December 2017, Pages 19-28
نویسندگان
, , , , , , ,