کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5514859 1541761 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas
چکیده انگلیسی


- Six mutations at Pro197 were found in multiple resistant corn poppy populations (groups O and B).
- No relation of phenotype and genotype unveiled non-target-site resistant mechanisms.
- Moreover, non-mutated plants in ALS gene survived imazamox treatments.
- Mobility of tribenuron-methyl in resistant plants indicated non-target-site resistant mechanisms.
- Enhanced imazamox metabolism was found in one multiple resistant population.

Target-site and non-target-site resistance mechanisms to ALS inhibitors were investigated in multiple resistant (tribenuron-methyl and 2,4-D) and only 2,4-D resistant, Spanish corn poppy populations. Six amino-acid replacements at the Pro197 position (Ala197, Arg197, His197, Leu197, Thr197 and Ser197) were found in three multiple resistant populations. These replacements were responsible for the high tribenuron-methyl resistance response, and some of them, especially Thr197 and Ser197, elucidated the cross-resistant pattern for imazamox and florasulam, respectively. Mutations outside of the conserved regions of the ALS gene (Gly427 and Leu648) were identified, but not related to resistance response. Higher mobility of labeled tribenuron-methyl in plants with multiple resistance was, however, similar to plants with only 2,4-D resistance, indicating the presence of non-target-site resistance mechanisms (NTSR). Metabolism studies confirmed the presence of a hydroxy imazamox metabolite in one of the populations. Lack of correlation between phenotype and genotype in plants treated with florasulam or imazamox, non-mutated plants surviving imazamox, tribenuron-methyl translocation patterns and the presence of enhanced metabolism revealed signs of the presence of NTSR mechanisms to ALS inhibitors in this species. On this basis, selection pressure with ALS non-SU inhibitors bears the risk of promoting the evolution of NTSR mechanisms in corn poppy.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 138, May 2017, Pages 57-65
نویسندگان
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