کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5515082 | 1400746 | 2017 | 4 صفحه PDF | دانلود رایگان |
BackgroundThe intracellular [Ca2+] is modulated by Ï receptors. An important component of the cellular machinery governing the intracellular [Ca2+] is Store-Operated Calcium Entry (SOCE). Here we want to investigate whether ligands of Ï receptors affect SOCE.MethodsThe intracellular [Ca2+] was monitored, with the fluorescent Ca2+-sensitive probe Fura-2, in four cell lines with a different expression of Ï receptors, namely MCF7 (expressing Ï1 receptors with a low density and overexpressing Ï2 receptors), MCF7Ï1 (overexpressing Ï1 receptors), SK-N-SH, and HT-29.ResultsWhen thapsigargin was used to deplete intracellular Ca2+ stores, in a Ca2+-free incubation medium, the Ca2+ influx (following Ca2+ re-addition) was significantly increased by 1 μM (+)-pentazocine (Ï1 receptor agonist) in MCF7Ï1 (by 22.5%) and SK-N-SH (by 45.6%), but not in HT-29 and MCF7 cells. We have used, as a second approach, the “Mn2+ quenching” protocol. In MCF7Ï1 cells, after thapsigargin treatment, the fluorescence quenching induced by Mn2+ influx (evidence of Ca2+ influx) was significantly increased (by 25.8%) by 1 μM (+)-pentazocine, significantly decreased (by 18.0%) by BD1063 (Ï1 receptor antagonist), and not affected by the presence of both ligands. These effects were not observed in MCF7 cells. Finally, in MCF7 cells, 1 μM PB28 (Ï2 receptor agonist), did not affect both the Ca2+ response after Ca2+ re-addition and the fluorescence quenching induced by Mn2+ influx.ConclusionsWe propose that the Ï1 receptor agonist (+)-pentazocine increases SOCE in MCF7Ï1 and SK-N-SH cell lines. The Ï2 receptor agonist PB28 does not affect SOCE in MCF7 cells.
Journal: Pharmacological Reports - Volume 69, Issue 3, June 2017, Pages 542-545