کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5518192 1543869 2017 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Asparagine slows down the breakdown of storage lipid and degradation of autophagic bodies in sugar-starved embryo axes of germinating lupin seeds
ترجمه فارسی عنوان
آسپاراژین باعث تخفیف چربی ذخیره سازی و تخریب بدنهای اتوفیزیک در محورهای جنین قندی گرسنه جوانه زده تخم لوپین می شود.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی

The research was conducted on embryo axes of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet), which were isolated from imbibed seeds and cultured for 96 h in vitro under different conditions of carbon and nitrogen nutrition. Isolated embryo axes were fed with 60 mM sucrose or were sugar-starved. The effect of 35 mM asparagine (a central amino acid in the metabolism of germinating lupin seeds) and 35 mM nitrate (used as an inorganic kind of nitrogen) on growth, storage lipid breakdown and autophagy was investigated. The sugar-starved isolated embryo axes contained more total lipid than axes fed with sucrose, and the content of this storage compound was even higher in sugar-starved isolated embryo axes fed with asparagine. Ultrastructural observations showed that asparagine significantly slowed down decomposition of autophagic bodies, and this allowed detailed analysis of their content. We found peroxisomes inside autophagic bodies in cells of sugar-starved Andean lupin embryo axes fed with asparagine, which led us to conclude that peroxisomes may be degraded during autophagy in sugar-starved isolated lupin embryo axes. One reason for the slower degradation of autophagic bodies was the markedly lower lipolytic activity in axes fed with asparagine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 209, February 2017, Pages 51-67
نویسندگان
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