کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5528799 | 1548550 | 2017 | 7 صفحه PDF | دانلود رایگان |
- A genome-wide screen for bisulfite sensitivity implicates RNA metabolism defects.
- Bisulfite impairs RNA dependant P-body formation.
- Bisulfite reduced RNA yields and blocked reporter gene expression in treated yeast.
Bisulfite, in the form of sodium bisulfite or metabisulfite, is used commercially as a food preservative. Bisulfite is used in the laboratory as a single-stranded DNA mutagen in epigenomic analyses of DNA methylation. Recently it has also been used on whole yeast cells to induce mutations in exposed single-stranded regions in vivo. To understand the effects of bisulfite on live cells we conducted a genome-wide screen for bisulfite sensitive mutants in yeast. Screening the deletion mutant array, and collections of essential gene mutants we define a genetic network of bisulfite sensitive mutants. Validation of screen hits revealed hyper-sensitivity of transcription and RNA processing mutants, rather than DNA repair pathways and follow-up analyses support a role in perturbation of RNA transactions. We propose a model in which bisulfite-modified nucleotides may interfere with transcription or RNA metabolism when used in vivo.
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 821, September 2017, Pages 13-19