کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530556 1549313 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of Ca2+ puffs evoked by extracellular agonists and photoreleased IP3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Comparison of Ca2+ puffs evoked by extracellular agonists and photoreleased IP3
چکیده انگلیسی


- GPCR agonists and photoreleased i-IP3 evoke Ca2+ puffs at overlapping sites.
- Puffs evoked by GPCR agonists and photoreleased i-IP3 have similar kinetics and spatial spread.
- Photoreleased i-IP3 faithfully mimics puffs evoked by the physiological IP3 pathway.
- Muscarinic and bradykinin receptors access the same IP3 receptors.

The inositol trisphosphate (IP3) signaling pathway evokes local Ca2+ signals (Ca2+ puffs) that arise from the concerted openings of clustered IP3 receptor/channels in the ER membrane. Physiological activation is triggered by binding of agonists to G-protein-coupled receptors (GPCRs) on the cell surface, leading to cleavage of phosphatidyl inositol bisphosphate and release of IP3 into the cytosol. Photorelease of IP3 from a caged precursor provides a convenient and widely employed means to study the final stage of IP3-mediated Ca2+ liberation, bypassing upstream signaling events to enable more precise control of the timing and relative concentration of cytosolic IP3. Here, we address whether Ca2+ puffs evoked by photoreleased IP3 fully replicate those arising from physiological agonist stimulation. We imaged puffs in individual SH-SY5Y neuroblastoma cells that were sequentially stimulated by picospritzing extracellular agonist (carbachol, CCH or bradykinin, BK) followed by photorelease of a poorly-metabolized IP3 analog, i-IP3. The centroid localizations of fluorescence signals during puffs evoked in the same cells by agonists and photorelease substantially overlapped (within ∼1 μm), suggesting that IP3 from both sources accesses the same, or closely co-localized clusters of IP3Rs. Moreover, the time course and spatial spread of puffs evoked by agonists and photorelease matched closely. Because photolysis generates IP3 uniformly throughout the cytoplasm, our results imply that IP3 generated in SH-SY5Y cells by activation of receptors to CCH and BK also exerts broadly distributed actions, rather than specifically activating a subpopulation of IP3Rs that are scaffolded in close proximity to cell surface receptors to form a signaling nanodomain.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 63, May 2017, Pages 43-47
نویسندگان
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