کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5534531 | 1551169 | 2017 | 8 صفحه PDF | دانلود رایگان |
- The tHDA-strip can be used to detect Salmonella in an easy way.
- The tHDA-strip can be used to detect Salmonella with high sensitivity.
- The whole detection procedures for real samples can be finished within 4-6Â h.
Salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. Since conventional culture methods to detect Salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. In this study, we developed a rapid, simple and portable Salmonella detection strategy that combines thermophilic helicase-dependent amplification (tHDA) with a lateral flow assay to provide a detection result based on visual signals within 90Â min. Performance analyses indicated that the method had detection limits for DNA and pure cultured bacteria of 73.4-80.7Â fg and 35-40Â CFU, respectively. Specificity analyses showed no cross reactions with Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Enterobacter aerogenes, Shigella and Campylobacter jejuni. The results for detection in real food samples showed that 1.3-1.9Â CFU/g or 1.3-1.9Â CFU/mL of Salmonella in contaminated chicken products and infant nutritional cereal could be detected after 2Â h of enrichment. The same amount of Salmonella in contaminated milk could be detected after 4Â h of enrichment. This tHDA-strip can be used for the rapid detection of Salmonella in food samples and is particularly suitable for use in areas with limited equipment.
Journal: Molecular and Cellular Probes - Volume 34, August 2017, Pages 37-44