کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5552450 1557946 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antiviral activity of a polysaccharide from Laminaria japonica against enterovirus 71
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Antiviral activity of a polysaccharide from Laminaria japonica against enterovirus 71
چکیده انگلیسی

This in vitro study investigated the antiviral activity of an acidic polysaccharide from Laminaria japonica against enterovirus 71 (EV71) as well as its mechanism of action. The LJ04 polysaccharide was purified from Laminaria japonica by affinity chromatography. To investigate its antiviral activity, an MTT assay, q-PCR, immunofluorescent staining and western-blot analysis were performed. To define its mechanism of action, ELISA, q-PCR and flow cytometry were conducted. LJ04 had a low EC50, high CC50 and high SI. LJ04 inhibited not only JN200804, but also JN200803 in RD cells, and viral proliferation was strongly inhibited, whereas LJ04 suppressed viral-induced apoptosis as detected by flow cytometry. In conclusion, LJ04 was found to have robust antiviral activity by inhibiting apoptosis and inducing IFN-β expression. Our findings indicate that LJ04 is a good candidate for the treatment of EV71.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 96, December 2017, Pages 256-262
نویسندگان
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