کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5560133 | 1403309 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Potato alkaloids (SC), PFOS and sucrose monolaurate (SML) studied in Caco-2 model.
- SC, PFOS and SML negatively affect tight junction (TJ) integrity.
- SC and SML negatively affect membrane integrity, but not PFOS.
- Surfactants acted additively on TJ integrity.
- Surfactant mixtures in the diet may put stress on the intestinal barrier function.
Surfactants may cause dysfunction of intestinal tight junctions (TJs), which is a common feature of intestinal autoimmune diseases. Effects of dietary surfactants on TJ integrity, measured as trans-epithelial resistance (TEER), were studied in Caco-2Â cell monolayers. Cytotoxicity was assessed as apical LDH leakage. Monolayers were apically exposed for 60Â min to the dietary surfactants solanine and chaconine (SC, potato glycoalkaloids, 0-0.25Â mM), perfluorooctane sulfonic acid (PFOS, industrial contaminant, 0-0.8Â mM), and sucrose monolaurate (SML, food emulsifier E 473, 0-2.0Â mM) separately and as a mixture. Dose-response modelling of TEER EC50 showed that SC were 2.7- and 12-fold more potent than PFOS and SML, respectively. The mixture was composed of 1Â molar unit SC, 2.7 units PFOS and 12 units SML (“SC TEER equivalent” proportions 1:1:1). Mixture exposure (0-0.05Â mM SC equivalents) dose-response modelling suggested additive action on TJ integrity. Increasing SC and SML concentrations caused increased LDH leakage, but PFOS decreased LDH leakage at intermediate exposure concentrations. In the mixture PFOS appeared to protect from extensive SC- and SML-induced LDH leakage. Complex mixtures of surfactants in food may act additively on intestinal TJ integrity, which should be considered in risk assessment of emulsifier authorisation for use in food production.
Journal: Food and Chemical Toxicology - Volume 106, Part A, August 2017, Pages 314-323