کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5588904 | 1569139 | 2017 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative transcriptome analysis reveals potential gene targets for ovipositional inhibition by diallyl trisulfide against female Sitotroga cerealella
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کلمات کلیدی
DEGsSitotroga cerealellaVDCChMTdiallyl trisulfideDATSGLDP450qRT-PCRSDRGSTCoGFecundity - باروری یا حاصلخیزیTakeout - بیرون بردنTranscriptome analysis - تجزیه و تحلیل تکراریcoding sequences - توالی کدگذاریOvarian development - توسعه تخمدانCluster of Orthologous Groups - خوشه گروه های OrthologousKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوShort-chain dehydrogenase/reductase - دلائل زنجیره ای دهیدروژناز / ردوکتازSwiss-Prot - سوئیس-پروتCytochrome P450 - سیتوکروم پی۴۵۰FIT - مناسبGene ontology - هستیشناسی ژنیJuvenile hormone - هورمون جوانیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیnon-redundant protein database - پایگاه داده پروتئین غیر انحصاریECi - پیاده رویDifferentially expressed genes - ژن های متفاوت بیان شده استCdS - کادمیم سولفید، سولفید کادمیمexperimental group - گروه تجربیcontrol group - گروه کنترلglutathione S-transferase - گلوتاتیون S-ترانسفرازGlucose dehydrogenase - گلوکز دی هیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The angoumois grain moth, Sitotroga cerealella Olivier, is a serious stored-grain pest worldwide. In previous studies, we demonstrated that diallyl trisulfide (DATS), one of active substances from garlic essential oil, inhibited oviposition of S. cerealella, but the mechanism for this inhibition remains unclear. We suspected that DATS-regulated gene expression changes in S. cerealella may be a reason for the modulation of fertility and reproductive behaviors of the moth. Comparative transcriptomes of female S. cerealella were built by RNA-sequencing to detect differences between DATS exposure and background air with special lights on the key genes related to the fecundity. A total of 304 differentially expressed genes (DEGs) were detected in female moth after exposure to DATS, of which 120 were up-regulated, and 184 were down-regulated. Sixty-two of these DEGs encoding proteins involved in growth and development of eggs and regulation of hormones and mating behavior were identified, and of these, 49 were annotated as chorion protein. Meanwhile, quantitative real-time PCR verified that DEG data were reliable for further transcriptional analysis. Additionally, the results of ovarian dissection and oviposition activity bioassay suggest that DATS has negative effects on ovarian development and fecundity of S. cerealella. Our comprehensive sequence resource with desirable quality enriched the genomic platform of S. cerealella, and the DEGs that were identified will facilitate understanding of the molecular mechanism of ovipositional inhibition by DATS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 5, September 2017, Pages 12-18
Journal: Agri Gene - Volume 5, September 2017, Pages 12-18
نویسندگان
Min Ma, Yi-Yi Ying, Zhi-Ya He, Yan Lu, Xue-Gang Li, Chao-Liang Lei, Feng-Lian Yang,