کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5591032 | 1570332 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conjugative type IV secretion in Gram-positive pathogens: TraG, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein TraM
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کلمات کلیدی
RBSMurNAcT4SSTMHSLTGlcNAcLC-MS/MS - LC-MS / MSN-acetylmuramic acid - N-استیلیمورمیک اسیدEnterococcus faecalis - انتروکوکوس فکالیسEndopeptidase - اندوپپتیدازlytic transglycosylase - ترانس گلیکوزیلاز خوراکیRibosomal binding site - سایت پیوند ریبوزومیType IV secretion system - سیستم ترشحی نوع IVtransmembrane helix - مارپیچ فرابنفشN-acetylglucosamine - نیتستیگلوکوزامینChap - چپliquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفهGram-negative - گرم منفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 transfer genes. In this work, we focus on the VirB1-like protein TraG, a modular peptidoglycan metabolizing enzyme, and the VirB8-homolog TraM, a potential member of the translocation channel. By providing full-length traG in trans, but not with a truncated variant, we achieved full recovery of wild type transfer efficiency in the traG-knockout mutant E. faecalis pIP501ÎtraG. With peptidoglycan digestion experiments and tandem mass spectrometry we could assign lytic transglycosylase and endopeptidase activity to TraG, with the CHAP domain alone displaying endopeptidase activity. We identified a novel interaction between TraG and TraM in a bacterial-2-hybrid assay. In addition we found that both proteins localize in focal spots at the E. faecalis cell membrane using immunostaining and fluorescence microscopy. Extracellular protease digestion to evaluate protein cell surface exposure revealed that correct membrane localization of TraM requires the transmembrane helix of TraG. Thus, we suggest an essential role for TraG in the assembly of the pIP501 type IV secretion system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 91, May 2017, Pages 9-18
Journal: Plasmid - Volume 91, May 2017, Pages 9-18
نویسندگان
Verena Kohler, Ines Probst, Andreas Aufschnaiter, Sabrina Büttner, Lisa Schaden, Gerald N. Rechberger, Günther Koraimann, Elisabeth Grohmann, Walter Keller,