کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5591826 1570701 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A point mutation in the extracellular domain of CD4 completely abolishes CD4 T cell development in C57BL/6 mouse
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
A point mutation in the extracellular domain of CD4 completely abolishes CD4 T cell development in C57BL/6 mouse
چکیده انگلیسی
In this study, we performed ENU mutagenesis and multi-parameter flow cytometric analysis in C57BL/6 mice to uncover novel genes or alleles regulating immune cell development. We identified a novel mutant allele of Cd4 gene which completely blocked development of a major subset of T cells named CD4 T cell. Our data for the first time showed experimentally in mice the critical role of the first extracellular domain, by obtaining mice with a loss of function mutation from Ile to Asn at the position 99 of CD4 (I99N). Interestingly, such CD4I99N mutant protein can be expressed on the surface of human cells, and the mRNA stability could be also affected by this point mutation, suggesting that absence of CD4 T cells in mice rooted in the deficiency in function and expression of CD4. In addition, we used this novel CD4 T cell deficient model as recipient mice for adoptive transfer experiment, and showed that it could be an optimal model for study of CD4 T cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 92, December 2017, Pages 12-20
نویسندگان
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