کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5629078 1580142 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research PaperEMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
Research PaperEMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex
چکیده انگلیسی


- SVZ-derived NPCs express MMPs and their physiological inducer, EMMPRIN.
- Transgenic overexpression of EMMPRIN enhances the enzymatic activity of MMP-2.
- EMMPRIN overexpression increases NPCs invasive capacities.
- NPCs overexpressing EMMPRIN migrate rapidly into cortical injury.

Stimulation of endogenous neurogenesis and recruitment of neural progenitors from the subventricular zone (SVZ) neurogenic site may represent a useful strategy to improve regeneration in the ischemic cortex. Here, we tested whether transgenic overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN), the regulator of matrix metalloproteinases (MMPs) expression, in endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) could increase migration towards ischemic injury. For this purpose, we applied a lentivector-mediated gene transfer system. We found that EMMPRIN-transduced progenitors exhibited enhanced MMP-2 activity in vitro and showed improved motility in 3D collagen gel as well as in cortical slices. Using a rat model of neonatal ischemia, we showed that EMMPRIN overexpressing SVZ cells invade the injured cortical tissue more efficiently than controls. Our results suggest that EMMPRIN overexpression could be suitable approach to improve capacities of endogenous or transplanted progenitors to invade the injured cortex.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 297, November 2017, Pages 14-24
نویسندگان
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