کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5649471 | 1407125 | 2016 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-203 Inversely Correlates with Differentiation Grade, Targets c-MYC, and Functions as a Tumor Suppressor in cSCC
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کلمات کلیدی
qPCRBCCODNISHCSCCEdU - EDUIn situ hybridization - Hybridization در محلQuantitative PCR - PCR کمیOligonucleotide - اولیگونوکلئوتیدUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNABasal cell carcinoma - کارسینوم سلول بازالcutaneous squamous cell carcinoma - کارسینوم سلول سنگفرشی پوستی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: MicroRNA-203 Inversely Correlates with Differentiation Grade, Targets c-MYC, and Functions as a Tumor Suppressor in cSCC MicroRNA-203 Inversely Correlates with Differentiation Grade, Targets c-MYC, and Functions as a Tumor Suppressor in cSCC](/preview/png/5649471.png)
چکیده انگلیسی
Cutaneous squamous cell carcinoma (cSCC) is the second most common cancer and a leading cause of cancer mortality among solid organ transplant recipients. MicroRNAs (miR) are short RNAs that regulate gene expression and cellular functions. Here, we show a negative correlation between miR-203 expression and the differentiation grade of cSCC. Functionally, miR-203 suppressed cell proliferation, cell motility, and the angiogenesis-inducing capacity of cSCC cells in vitro and reduced xenograft tumor volume and angiogenesis in vivo. Transcriptomic analysis of cSCC cells with ectopic overexpression of miR-203 showed dramatic changes in gene networks related to cell cycle and proliferation. Transcription factor enrichment analysis identified c-MYC as a hub of miR-203-induced transcriptomic changes in squamous cell carcinoma. We identified c-MYC as a direct target of miR-203. Overexpression of c-MYC in rescue experiments reversed miR-203-induced growth arrest in cSCC, which highlights the importance of c-MYC within the miR-203-regulated gene network. Together, miR-203 acts as a tumor suppressor in cSCC, and its low expression can be a marker for poorly differentiated tumors. Restoration of miR-203 expression may provide a therapeutic benefit, particularly in poorly differentiated cSCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 12, December 2016, Pages 2485-2494
Journal: Journal of Investigative Dermatology - Volume 136, Issue 12, December 2016, Pages 2485-2494
نویسندگان
Warangkana Lohcharoenkal, Masako Harada, Jakob Lovén, Florian Meisgen, Ning Xu Landén, Lingyun Zhang, Jan Lapins, Kunal Das Mahapatra, Hao Shi, Liisa Nissinen, Veli-Matti Kähäri, Mona StÃ¥hle, Enikö Sonkoly, Dan Grandér, Marie Arsenian-Henriksson,