کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
566189 1367708 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High resolution ultrasound and photoacoustic imaging of single cells
ترجمه فارسی عنوان
تصویربرداری با سونوگرافی با وضوح بالا و عکس برداری از سلولهای تک سلولی
کلمات کلیدی
میکروسکوپ آکوستیک؛ میکروسکوپ فوتوکوسیت؛ لکه خون؛ لکوسیت ها
موضوعات مرتبط
مهندسی و علوم پایه شیمی طیف سنجی
چکیده انگلیسی


• Single leukocytes were imaged using ultrasound and photoacoustic microscopy.
• A fiber-coupled pulsed Nd:YAG laser enabled multispectral photoacoustic imaging.
• The nuclei of neutrophils, lymphocytes and monocytes were visible with ultrasound.
• Cellular features were enhanced using different photoacoustic excitation wavelengths.
• Neutrophils, lymphocytes and monocytes were identified from the generated images.

High resolution ultrasound and photoacoustic images of stained neutrophils, lymphocytes and monocytes from a blood smear were acquired using a combined acoustic/photoacoustic microscope. Photoacoustic images were created using a pulsed 532 nm laser that was coupled to a single mode fiber to produce output wavelengths from 532 nm to 620 nm via stimulated Raman scattering. The excitation wavelength was selected using optical filters and focused onto the sample using a 20× objective. A 1000 MHz transducer was co-aligned with the laser spot and used for ultrasound and photoacoustic images, enabling micrometer resolution with both modalities. The different cell types could be easily identified due to variations in contrast within the acoustic and photoacoustic images. This technique provides a new way of probing leukocyte structure with potential applications towards detecting cellular abnormalities and diseased cells at the single cell level.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photoacoustics - Volume 4, Issue 1, March 2016, Pages 36–42
نویسندگان
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