کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665939 1407778 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis
ترجمه فارسی عنوان
تشخیص سیتومگالوویروس انسان در سرطان معده و ارتباط احتمالی آن با متاستاز لنفاوی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- No difference of HCMV seropositivity between the GC patients and the healthy.
- Higher prevalence of HCMV in GC tissues than in adjacent normal tissues.
- Higher HCMV load is in GC tissues than in adjacent normal tissues.
- HCMV load in GC tissues is positively associated with GC lymphatic metastasis.

Increasing evidence suggests that human cytomegalovirus (HCMV) is associated with many human malignancies. However, its prevalence in gastric cancer (GC) and clinical association remain unknown. HCMV IgG and IgM antibodies in the sera of 80 GC patients and 80 healthy controls were detected using a microparticle enzyme immunoassay. The prevalence of HCMV UL47, UL55, UL56, and UL77 genes among 102 GC tumor tissues and adjacent normal specimens was measured by polymerase chain reaction (PCR) or nested PCR. Quantitative real-time PCR (Q-PCR) was used to determine viral load. Virus localization in neoplastic tissues was determined by immunohistochemistry. No significant difference of HCMV IgG and IgM seropositivity was found between GC patients and the healthy group. However, the overall HCMV DNA positivity rate was significantly higher in GC cancerous tissue compared with in paired normal tissue (P < 0.01). HCMV infection was mainly localized in the tumorous epithelium. Q-PCR in HCMV-positive specimens indicated that the viral copy number was notably higher in GC tissues than in adjacent normal specimens (P < 0.001). Clinical statistical analysis indicated that HCMV load in GC tumor tissue was positively associated with lymphatic metastasis (P = 0.043), the area under the receiver operating characteristic (ROC) curve was 0.6638. Our data clearly provide the prevalence of HCMV in GC patients. We conclude that HCMV infection in malignant tissues might be associated with carcinogenesis or progression of GC and possibly relates to lymphatic metastasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 88, Issue 1, May 2017, Pages 62-68
نویسندگان
, , , , , , , , , ,