کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5666651 | 1591535 | 2017 | 7 صفحه PDF | دانلود رایگان |
- We identify a novel CD8αα+ TCRαβ+ IEL subset.
- Thy1.2 is a marker for a novel CD8αα+ TCRαβ+ IEL subset.
- Thy1.2+ CD8αα+ TCRαβ+ IELs are generated from CD8α+ thymic precursors.
Intraepithelial lymphocytes (IELs) are resident cells localized within the intestinal epithelia and play an important role in regulating gut inflammations and host defense against pathogens. CD8α+ TCRαβ+ IELs are heterogeneous populations that are generated from T cell precursors including CD4â CD8αâ double-negative (DN) cells and CD4+ CD8α+ double-positive (DP) cells. However, developmental pathways of TCRαβ+ IELs remained unclear. To gain insight into the mechanisms, we generated mice (Bcl11bÎDN2 mice) that lack thymic precursors (DN CD5+ TCRβ+ cells) for CD4â CD8αα+ TCRαβ+ IELs. Unexpectedly, we found that, in the absence of the precursors in thymi of Bcl11bÎDN2 mice, CD4â CD8αα+ TCRαβ+ IELs were still present in the intestine though the number was reduced. Adoptive transfer experiment showed that their precursors were highly enriched in CD8α+ TCRβâ thymocytes. The CD4â CD8αα+ TCRαβ+ IELs in Bcl11bÎDN2 mice are distinguished by Thy1.2 expression and are indeed present in WT mice. Taken together, our study reveal a novel developmental pathway for CD8αα+ TCRαβ+ IELs.
Journal: Immunology Letters - Volume 191, November 2017, Pages 40-46