کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5668130 1592336 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pitfalls in interpretation of CT-values of RT-PCR in children with acute respiratory tract infections
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Pitfalls in interpretation of CT-values of RT-PCR in children with acute respiratory tract infections
چکیده انگلیسی

BackgroundThe relation between viral load and disease severity in childhood acute respiratory tract infections (ARI) is not fully understood.ObjectivesTo assess the clinical relevance of the relation between viral load, determined by cycle threshold (CT) value of real-time reverse transcription-polymerase chain reaction assays and disease severity in children with single- and multiple viral ARI.Study design582 children with ARI were prospectively followed and tested for 15 viruses. Correlations were calculated between CT values and clinical parameters.ResultsIn single viral ARI, statistically significant correlations were found between viral loads of Respiratory Syncytial Virus (RSV) and hospitalization and between viral loads of Human Coronavirus (HCoV) and a disease severity score. In multiple-viral ARI, statistically significant correlations between viral load and clinical parameters were found. In RSV-Rhinovirus (RV) multiple infections, a low viral load of RV was correlated with a high length of hospital stay and a high duration of extra oxygen use. The mean CT value for RV, HCoV and Parainfluenza virus was significantly lower in single- versus multiple infections.ConclusionAlthough correlations between CT values and clinical parameters in patients with single and multiple viral infection were found, the clinical importance of these findings is limited because individual differences in host-, viral and laboratory factors complicate the interpretation of statistically significant findings. In multiple infections, viral load cannot be used to differentiate between disease causing virus and innocent bystanders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 90, May 2017, Pages 1-6
نویسندگان
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