کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5670985 1408037 2018 8 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Discovery of human scFvs that cross-neutralize the toxic effects of B. jararacussu and C. d. terrificus venoms
کلمات کلیدی
Bothrops jararacussu; Crotalus durissus terrificus; scFv; Antivenom; Phage display; Indirect hemolysis; Plasma-clotting;
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Discovery of human scFvs that cross-neutralize the toxic effects of B. jararacussu and C. d. terrificus venoms
چکیده انگلیسی

•Human scFvs were selected against Bothrops jararacussu and Crotalus durissius terrificus venoms using Phage Display.•Human scFvs neutralize the hemolytic activity of crotalic and bothropic venoms.•Human scFvs neutralize plasma-clotting activity of crotalic and bothropic venoms.•Human scFvs prolonged survival of envenomed animals.

Accidents involving venomous snakes are a public health problem worldwide, causing a large number of deaths per year. In Brazil, the majority of accidents are caused by the Bothrops and Crotalus genera, which are responsible for approximately 80% of severe envenoming cases. The cross-neutralization of snake venoms by antibodies is an important issue for development of more effective treatments. Our group has previously reported the construction of human monoclonal antibody fragments towards Bothrops jararacussu and Crotalus durissus terrificus’ venoms. This study aimed to select human single-chain variable fragments (scFvs) that recognize both bothropic and crotalic crude venoms following venoms neutralizing capacity in vitro and in vivo. The cross-reactivity of Cro-Bothrumabs were demonstrated by ELISA and in vitro and in vivo experiments showed that a combination of scFvs neutralizes in vitro toxic activities (e.g. indirect hemolysis and plasma-clotting) of crotalic and bothropic venoms as well as prolonged survival time of envenomed animals. Our results may contribute to the development of the first human polyvalent antivenom against Bothrops jararacussu and Crotalus durissus terrificus venoms, overcoming some undesirable effects caused by conventional serotherapy.

Graphical abstractDownload high-res image (121KB)Download full-size image

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 177, January 2018, Pages 66-73
نویسندگان
, , , , , , ,