کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5796599 | 1555010 | 2016 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evidence for the existence of regulatory and effector B cell populations in Peyer's patches of sheep Evidence for the existence of regulatory and effector B cell populations in Peyer's patches of sheep](/preview/png/5796599.png)
IL-10 secreting CD21+ B cells exist in sheep Peyer's patches (PP). It's not known however, whether all PP B cells are regulatory or whether an effector population also exists in this tissue. To further characterize the subpopulations of B cells in PP's, highly purified B cells were negatively sorted from jejunal PP and fractionated according to co-expression of CD72+CD21+or CD72+CD21â molecules and then stimulated with the TLR9-agonist, CpG ODN. IL-10, IL-12, IFN-γ, and IgM production were then assayed. We observed that only highly purified CD72+CD21+ B cells spontaneously secreted high levels of IL-10, but they did not produce any IL-12, IFN-γ or IgM suggesting that this cell population contains regulatory B cells. In contrast, CD72+CD21â B cells did not secrete IL-10, but secreted IL-12, IFN-γ, and IgM, suggesting they include effector cells. In addition, B cells expressing surface IgA, IgM and IgG1 all secreted similar levels of IL-10. We further confirmed that only B cells produce IL-10, while other cells in the PP including DCs and T cells do not. Our investigations may provide evidence for the existence of two sub-populations in sheep PP; IL-10 secreting regulatory (CD72+CD21+) cells, and IL-12/IFN-γ/IgM-secreting effector (CD72+CD21â) cells.
Journal: Veterinary Immunology and Immunopathology - Volume 174, June 2016, Pages 26-34