کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5823983 | 1118383 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The food colorant erythrosine is a promiscuous protein-protein interaction inhibitor
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کلمات کلیدی
EGFDAPIHRPPPIIC50FBSTNFPBS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBSA - BSAADI - NAMEbovine serum albumin - آلبومین سرم گاوErythrosine - اریتروستینBAFF - بافBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینProtein–protein interaction - تعامل پروتئین-پروتئینTNF superfamily - خانواده TNFAcceptable daily intake - دریافت روزانه قابل قبولRoom temperature - دمای اتاقFood color - رنگ غذاXanthene dyes - رنگ های زانتیfetal bovine serum - سرم جنین گاوTHP-1 cells - سلول های THP-1epidermal growth factor - عامل رشد اپیدرمیmedian inhibitory concentration - غلظت بازدارنده متوسطtumor necrosis factor - فاکتور نکروز تومورPhosphate buffered saline - فسفات بافر شورHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Following our observation that erythrosine B (FD&C Red No. 3) is a relatively potent inhibitor of the TNF-R-TNFα and CD40-CD154 protein-protein interactions, we investigated whether this inhibitory activity extends to any other protein-protein interactions (PPI) as well as whether any other approved food colors possess such inhibitory activity. We found erythrosine, a poly-iodinated xanthene dye, to be a non-specific promiscuous inhibitor of a number of PPIs within the tumor necrosis factor superfamily (TNF-R-TNFα, CD40-CD154, BAFF-R-BAFF, RANK-RANKL, OX40-OX40L, 4-1BB-4-1BBL) as well as outside of it (EGF-R-EGF) with a remarkably consistent median inhibitory concentration (IC50) in the 2-20 μM (approximately 2-20 mg/L) range. In agreement with this, erythrosine also showed cellular effects including clear cytotoxic effects around this concentration range (IC50 â 50 μM). Among the seven FDA-approved food colorants, only erythrosine showed consistent PPI inhibitory activity in the sub-100 μM range, which might also explain (at least partially) why it also has the lowest approved acceptable daily intake (ADI) (0.1 mg/kg body weight/day). Among a number of xanthene structural analogs of erythrosine tested for activity, rose Bengal, a food colorant approved in Japan, showed similar, maybe even more pronounced, promiscuous inhibitory activity, whereas fluorescein was inactive and gallein, phloxine, and eosin were somewhat active in some of the assays.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 81, Issue 6, 15 March 2011, Pages 810-818
Journal: Biochemical Pharmacology - Volume 81, Issue 6, 15 March 2011, Pages 810-818
نویسندگان
Lakshmi Ganesan, Emilio Margolles-Clark, Yun Song, Peter Buchwald,