Characterization of the cDNA and in vitro expression of the ram seminal plasma protein RSVP14

► We have characterized and expressed the ram seminal plasma protein RSVP14 gene. ► The cDNA sequence shows numerous biochemical similarities with BSP proteins. ► One CRAC and two FN-2 domains suggest a role for RSVP14 in capacitation. ► The results might explain the RSVP14 protective effect against cold-shock injury. ► The recombinant protein was obtained in insect cell extracts and E. coli.