کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5913820 1162703 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Compaction of isolated Escherichia coli nucleoids: Polymer and H-NS protein synergetics
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Compaction of isolated Escherichia coli nucleoids: Polymer and H-NS protein synergetics
چکیده انگلیسی

Escherichia coli nucleoids were compacted by the inert polymer polyethylene glycol (PEG) in the presence of the H-NS protein. The protein by itself appears to have little impact on the size of the nucleoids as determined by fluorescent microscopy. However, it has a significant impact on the nucleoidal collapse by PEG. This is quantitatively explained by assuming the H-NS protein enhances the effective diameter of the DNA helix leading to an increase in the depletion forces induced by the PEG. Ultimately, however, the free energy of the nucleoid itself turns out to be independent of the H-NS concentration. This is because the enhancement of the supercoil excluded volume is negligible. The experiments on the nucleoids are corroborated by dynamic light scattering and EMSA analyses performed on DNA plasmids in the presence of PEG and H-NS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 194, Issue 1, April 2016, Pages 129-137
نویسندگان
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