کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914383 | 1162734 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alternative binding modes of l-histidine guided by metal ions for the activation of the antiterminator protein HutP of Bacillus subtilis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
Anti-terminator proteins control gene expression by recognizing control signals within cognate transcripts and then preventing transcription termination. HutP is such a regulatory protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences in hut mRNAs. During the anti-termination process, l-histidine and a divalent ion are required for hutP to bind to the specific sequence within the hut mRNA. Our previous crystal structure of the HutP-l-histidine-Mg2+-RNA ternary complex demonstrated that the l-histidine ligand and Mg2+ bind together such that the backbone nitrogen and carboxyl oxygen of l-histidine coordinate with Mg2+. In addition to the Mg2+, other divalent ions are also known to efficiently support the l-histidine-dependent anti-termination of the hut operon, and the best divalent ion is Zn2+. In this study, we determined the crystal structure of the HutP-l-histidine-Zn2+ complex and found that the orientation of l-histidine coordinated to Zn2+ is reversed relative to that of l-histidine coordinated to Mg2+, i.e., the imidazole side chain nitrogen of l-histidine coordinates to Zn2+. This alternative binding mode of the l-histidine ligand to a divalent ion provides further insight into the mechanisms responsible for the activation of RNA binding during the hut anti-termination process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 183, Issue 3, September 2013, Pages 512-518
Journal: Journal of Structural Biology - Volume 183, Issue 3, September 2013, Pages 512-518
نویسندگان
Balasundaresan Dhakshnamoorthy, Hiroshi Mizuno, Penmetcha K.R. Kumar,