Establishment of a transient transfection system and expression of firefly luciferase in Entamoeba invadens

► Entamoeba invadens is the model organism for Entamoeba histolytica. ► In this study we have reported the transient transfection method for E. invadens. ► Luciferase gene was used as a reporter in this study. ► We used promoter of E. invadens ribosomal protein gene for luciferase gene expression.