کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5917646 | 1570742 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nucleotide oligomerization domain-2 interacts with 2â²-5â²-oligoadenylate synthetase type 2 and enhances RNase-L function in THP-1 cells
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کلمات کلیدی
Calmodulin binding peptideSBPNLRsRLRsCBPOAS2muramyl dipeptideNOD2MDPTLRs - TLR هاInnate immunity - ایمنی ذاتیcaspase activation and recruitment domain - فعال سازی caspase و دامنه استخدامOAS - واحهProteomics - پروتئومیکسpoly(I:C) - پلی (I: C)CARD - کارتVector control - کنترل بردارToll-like receptors - گیرنده های پولی مانند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nucleotide-binding and oligomerization domain-2 (NOD2) is an intracellular protein involved in innate immunity and linked to chronic inflammatory diseases in humans. Further characterization of the full spectrum of proteins capable of binding to NOD2 may provide new insights into its normal functioning as well as the mechanisms by which mutated forms cause disease. Using a proteomics approach to study human THP-1 cells, we have identified 2â²-5â²-oligoadenylate synthetase type 2 (OAS2), a dsRNA binding protein involved in the pathway that activates RNase-L, as a new binding partner for NOD2. The interaction was confirmed using over-expression of OAS2 and NOD2 in HEK cells. Further confirmation was obtained by detecting NOD2 in immunoprecipitates of endogenous OAS2 in THP-1 cells. Finally, over-expression of NOD2 in THP-1 cells led to enhanced RNase-L activity in cells treated with poly(I:C), a mimic of double-stranded RNA virus infection. These data indicate connectivity in pathways involved in innate immunity to bacteria and viruses and suggest a regulatory role whereby NOD2 enhances the function of RNase-L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 47, Issues 2â3, December 2009, Pages 560-566
Journal: Molecular Immunology - Volume 47, Issues 2â3, December 2009, Pages 560-566
نویسندگان
Jae W. Dugan, Amador Albor, Larry David, Jonathan Fowlkes, Marc T. Blackledge, Tammy M. Martin, Stephen R. Planck, Holly L. Rosenzweig, James T. Rosenbaum, Michael P. Davey,