Direct thermal noise calibration of colloidal probe cantilevers

- The order of “calibration” and “particle attachment” is irrelevant.
- The equipartition theorem is applicable to colloidal probes as well.
- The material and the size of the microparticles used made no difference.
- The cantilevers spring constant, shape or dimensions showed no influence.
- Advice on preparation to increase reliability and reproducibility is suggested.