کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599291 1454269 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biodegradable and versatile polyethylenimine derivatives efficiently transfer DNA and siRNA into mammalian cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Biodegradable and versatile polyethylenimine derivatives efficiently transfer DNA and siRNA into mammalian cells
چکیده انگلیسی


• The strategy is simple and involves a two-step synthesis protocol, viz., aza-Michael addition and amidation.
• Biodegradable vectors based on low molecular weight PEIs were obtained in quantitative yields.
• Both the vectors efficiently condensed nucleic acids and delivered into the mammalian cells
• Transfection efficiency of the projected vectors was several folds higher than the native polymers and the commercially available Lipofectamine 2000 and Superfect without imparting toxicity.
• Both the vectors efficiently delivered siRNA.

Polyethylenimines (PEIs) are considered as the most promising vectors for non-viral gene delivery applications. Here, we report the synthesis and in vitro evaluation of two non-toxic and biodegradable polymers, TEPA@bPEI (TBP) and TEPA@lPEI (TLP), derived from low molecular weight branched and linear polyethylenimines by the stepwise reactions with methylacrylate (aza-Michael reaction) and amidation with tetraethylenepentamine (TEPA). These polymers not only showed their ability to bind and condense pDNA into nano-sized complexes but also provided protection against nucleases in cellular milieu. Both the polymers exhibited excellent buffering capacity and efficiently delivered nucleic acids (plasmid DNA and siRNA) across the mammalian cells (CHO and A549 cells) and outclassed native polymers and the commercial transfection reagents in terms of transfection efficiency and target gene silencing, and that too without compromising on biocompatibility i.e. toxicity. The results advocate the promising potential of the PEI derivatives as safe and potent nucleic acid carriers for practical gene delivery applications.

Two new non-toxic and biodegradable polymers, TEPA@bPEI (TBP) and TEPA@lPEI (TLP), are synthesized, which efficiently deliver nucleic acids in vitro outcompeting native polymers and the commercial vectors in terms of transfection efficacy.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 135, 1 November 2015, Pages 661–668
نویسندگان
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