کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
600922 | 1454314 | 2012 | 5 صفحه PDF | دانلود رایگان |
Human mesenchymal stem cells (hMSCs) are multipotent and have been recognized as a source for tissue engineering or cell therapy. It is, therefore, imperative to develop methods to acquire enough hMSCs that maintain self-renewal and differentiation potential. However, aged hMSCs are prone to have a gradual decline in differentiation and proliferation potential with continual cell cycle divisions during in vitro culture. The physiochemical properties of hMSCs are highly dependent on their micro-environment, i.e. the ‘stem cell niche’. In this study, the heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces. Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression. High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans. These results will prove valuable to the design of scaffolds for tissue engineering or for the modulation of implantation environments.
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► The heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces.
► Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression.
► High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 90, 1 February 2012, Pages 36–40